|Intracytoplasmic Sperm Injection|
The Intracytoplasmic Sperm Injection (or ICSI) is a micromanipulation technique that allows the embryologist to inject a single sperm into a mature oocyte. By doing so, he or she ensures that the embryo is penetrated by a sperm, leaving nothing to chance.
Firstly, both the oocyte and sperm are prepared by removing any unwanted material. Once cleaned, the oocyte is placed within a holding dish separate from the washed sperm. The embryologist then assorts the good sperm according to their motility (movement) and morphology (appearance) into a fine glass capillary. The oocyte is then held in place by another capillary through suction, and the sperm is carefully injected into the centre of the oocyte. After the injections are finished, oocytes are placed back in culture media and incubated.
ICSI is utilized in cases where patients are presented with an abnormally low sperm count, when surgical techniques are required to obtain sperm or when there are very few oocytes that have the potential to be fertilized.